Trial of nested PCR by using DNA templates extracted from preparations for microscopic tests for cryptosporidium oocysts and Giardia cysts.
نویسندگان
چکیده
منابع مشابه
Evaluation of immunomagnetic separation and the sucrose flotation methods coupled with immunofluorescence or PCR for detection of Cryptosporidium and Giardia (oo)cysts in water samples
Introduction: Detection of Cryptosporidium and Giardia parasites in water samples is usually performed by US Environmental Protection Agency 1623 method. Nevertheless, the USEPA1623 method still need improvement, to prevent and control the water borne parasitic disease. Therefore, we undertook the present study. Materials and methods: Totally 48 surface water samples were collected. Four sampl...
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BACKGROUND The protozoan parasites Cryptosporidium spp. and Giardia are known to occur widely in both raw and drinking waters. They are two of the causative agents of waterborne outbreaks of gastroenteritis throughout the world. In the present study, a PCR assay and FA were developed for detection of Cryptosporidium oocysts and Giardia cyst in environmental samples. METHODS We have detected C...
متن کاملDNA Extraction from Protozoan Oocysts/Cysts in Feces for Diagnostic PCR
PCR detection of intestinal protozoa is often restrained by a poor DNA recovery or by inhibitors present in feces. The need for an extraction protocol that can overcome these obstacles is therefore clear. QIAamp® DNA Stool Mini Kit (Qiagen) was evaluated for its ability to recover DNA from oocysts/cysts directly from feces. Twenty-five Giardia-positive, 15 Cryptosporidium-positive, 15 Entamoeba...
متن کاملImmunomagnetic capture PCR to detect viable Cryptosporidium parvum oocysts from environmental samples.
A method to detect viable Cryptosporidium parvum oocysts was developed. Polyclonal immunoglobulin G against C. parvum oocyst and sporozoite surface antigens was purified from rabbit immune serum, biotinylated, and bound to streptoavidin-coated magnetic particles. C. parvum oocysts were captured by a specific antigen-antibody reaction and magnetic separation. The oocysts were then induced to exc...
متن کاملGiardia and Cryptosporidium in harp and hooded seals from the Gulf of St. Lawrence, Canada.
Giardia and Cryptosporidium are protozoan parasites known to cause enteric disease in terrestrial wildlife species (mammals, reptiles and birds). Few surveys for Giardia and Cryptosporidium in marine wildlife species, such as pinnipeds, have been reported. The objective of this study was to determine the prevalence and genotype of Giardia and Cryptosporidium in two species of pinnipeds, harp se...
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ورودعنوان ژورنال:
- Japanese journal of infectious diseases
دوره 59 4 شماره
صفحات -
تاریخ انتشار 2006